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Promega plasmid pcat-control
Plasmid Pcat Control, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+pcat-control/pcat+basic+plasmid/pmc06157379-66-1-3
Average 90 stars, based on 1 article reviews
plasmid pcat-control - by Bioz Stars, 2026-10
90/100 stars

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Plasmid Preparation:

Article Title: Understanding oligonucleotide-mediated inhibition of gene expression in Xenopus laevis oocytes
Article Snippet: Preparation of plasmid pCAT-control was purchased from Promega Biotech (Madison, WI). pCAT-control and all other plasmids used were amplified and purified after transformation into Escherichia coli strain DH5-α, using a Qiagen (Chatsworth, CA) midi preparation kit.

Article Title: Suppression of interleukin-1beta-induced nitric-oxide synthase promoter/enhancer activity by transforming growth factor-beta1 in vascular smooth muscle cells. Evidence for mechanisms other than NF-kappaB.
Article Snippet: Plasmid pCAT-Control contained the prokaryotic chloramphenicol acetyltransferase (CAT) gene (Promega) driven by the SV40 promoter and enhancer.

Article Title: Tumor specific promoters of the midkine gene that allow for selective expression in P53-inactivated cells
Article Snippet: Examination on Transcription Activity by Tumor-specific Promoters Derived From the Midkine Gene Using the above plasmid, or a plasmid pCAT-Control (manufactured by Promega) having the promoter of SV40 virus, and a plasmid pCAT-Basic (manufactured by Promega) containing no promoters, gene introduction was performed on the lung cancer cells QG-56, the neuroblastoma NGP, and the normal human fibroblasts MRC-5 that were being cultured in a DMEM (Sigma) supplemented with 10% bovine fetal serum.

Article Title: Use of the human MDR1 promoter for heat-inducible expression of therapeutic genes.
Article Snippet: The promoter of the human multidrug resistance gene (mdr1) harbors stress-responsive elements, which can be induced e.g., by heat or cytostatic drugs.. In previous studies the drug-responsiveness of the mdr1 promoter was successfully used for the drug-inducible expression of the human TNFgene in vitro and in vivo.. Beside the drug-responsive elements of the mdr1 promoter, heat-shock responsive elements have also been identified, which could be exploited for construction of heat-inducible expression vectors.

Article Title: Effect of Adenovirus E1A on ICAM-1 Promoter Activity in Human Alveolar and Bronchial Epithelial Cells
Article Snippet: The plasmid pCAT-Control (Promega, Madison, WI), which contains the SV40 promoter and enhancer sequences placed 5’ of the CAT gene, was used as a positive control for CAT expression and as a means of monitoring differences in transfection efficiency between cell lines.

Positive Control:

Article Title: Understanding oligonucleotide-mediated inhibition of gene expression in Xenopus laevis oocytes
Article Snippet: Preparation of plasmid pCAT-control was purchased from Promega Biotech (Madison, WI). pCAT-control and all other plasmids used were amplified and purified after transformation into Escherichia coli strain DH5-α, using a Qiagen (Chatsworth, CA) midi preparation kit.

Article Title: Suppression of interleukin-1beta-induced nitric-oxide synthase promoter/enhancer activity by transforming growth factor-beta1 in vascular smooth muscle cells. Evidence for mechanisms other than NF-kappaB.
Article Snippet: Plasmid pCAT-Control contained the prokaryotic chloramphenicol acetyltransferase (CAT) gene (Promega) driven by the SV40 promoter and enhancer.

Article Title: Tumor specific promoters of the midkine gene that allow for selective expression in P53-inactivated cells
Article Snippet: Examination on Transcription Activity by Tumor-specific Promoters Derived From the Midkine Gene Using the above plasmid, or a plasmid pCAT-Control (manufactured by Promega) having the promoter of SV40 virus, and a plasmid pCAT-Basic (manufactured by Promega) containing no promoters, gene introduction was performed on the lung cancer cells QG-56, the neuroblastoma NGP, and the normal human fibroblasts MRC-5 that were being cultured in a DMEM (Sigma) supplemented with 10% bovine fetal serum.

Article Title: Use of the human MDR1 promoter for heat-inducible expression of therapeutic genes.
Article Snippet: The promoter of the human multidrug resistance gene (mdr1) harbors stress-responsive elements, which can be induced e.g., by heat or cytostatic drugs.. In previous studies the drug-responsiveness of the mdr1 promoter was successfully used for the drug-inducible expression of the human TNFgene in vitro and in vivo.. Beside the drug-responsive elements of the mdr1 promoter, heat-shock responsive elements have also been identified, which could be exploited for construction of heat-inducible expression vectors.

Article Title: Effect of Adenovirus E1A on ICAM-1 Promoter Activity in Human Alveolar and Bronchial Epithelial Cells
Article Snippet: The plasmid pCAT-Control (Promega, Madison, WI), which contains the SV40 promoter and enhancer sequences placed 5’ of the CAT gene, was used as a positive control for CAT expression and as a means of monitoring differences in transfection efficiency between cell lines.

Expressing:

Article Title: Understanding oligonucleotide-mediated inhibition of gene expression in Xenopus laevis oocytes
Article Snippet: Preparation of plasmid pCAT-control was purchased from Promega Biotech (Madison, WI). pCAT-control and all other plasmids used were amplified and purified after transformation into Escherichia coli strain DH5-α, using a Qiagen (Chatsworth, CA) midi preparation kit.

Article Title: Suppression of interleukin-1beta-induced nitric-oxide synthase promoter/enhancer activity by transforming growth factor-beta1 in vascular smooth muscle cells. Evidence for mechanisms other than NF-kappaB.
Article Snippet: Plasmid pCAT-Control contained the prokaryotic chloramphenicol acetyltransferase (CAT) gene (Promega) driven by the SV40 promoter and enhancer.

Article Title: Tumor specific promoters of the midkine gene that allow for selective expression in P53-inactivated cells
Article Snippet: Examination on Transcription Activity by Tumor-specific Promoters Derived From the Midkine Gene Using the above plasmid, or a plasmid pCAT-Control (manufactured by Promega) having the promoter of SV40 virus, and a plasmid pCAT-Basic (manufactured by Promega) containing no promoters, gene introduction was performed on the lung cancer cells QG-56, the neuroblastoma NGP, and the normal human fibroblasts MRC-5 that were being cultured in a DMEM (Sigma) supplemented with 10% bovine fetal serum.

Article Title: Use of the human MDR1 promoter for heat-inducible expression of therapeutic genes.
Article Snippet: The promoter of the human multidrug resistance gene (mdr1) harbors stress-responsive elements, which can be induced e.g., by heat or cytostatic drugs.. In previous studies the drug-responsiveness of the mdr1 promoter was successfully used for the drug-inducible expression of the human TNFgene in vitro and in vivo.. Beside the drug-responsive elements of the mdr1 promoter, heat-shock responsive elements have also been identified, which could be exploited for construction of heat-inducible expression vectors.

Article Title: Effect of Adenovirus E1A on ICAM-1 Promoter Activity in Human Alveolar and Bronchial Epithelial Cells
Article Snippet: The plasmid pCAT-Control (Promega, Madison, WI), which contains the SV40 promoter and enhancer sequences placed 5’ of the CAT gene, was used as a positive control for CAT expression and as a means of monitoring differences in transfection efficiency between cell lines.

Transfection:

Article Title: Understanding oligonucleotide-mediated inhibition of gene expression in Xenopus laevis oocytes
Article Snippet: Preparation of plasmid pCAT-control was purchased from Promega Biotech (Madison, WI). pCAT-control and all other plasmids used were amplified and purified after transformation into Escherichia coli strain DH5-α, using a Qiagen (Chatsworth, CA) midi preparation kit.

Article Title: Suppression of interleukin-1beta-induced nitric-oxide synthase promoter/enhancer activity by transforming growth factor-beta1 in vascular smooth muscle cells. Evidence for mechanisms other than NF-kappaB.
Article Snippet: Plasmid pCAT-Control contained the prokaryotic chloramphenicol acetyltransferase (CAT) gene (Promega) driven by the SV40 promoter and enhancer.

Article Title: Tumor specific promoters of the midkine gene that allow for selective expression in P53-inactivated cells
Article Snippet: Examination on Transcription Activity by Tumor-specific Promoters Derived From the Midkine Gene Using the above plasmid, or a plasmid pCAT-Control (manufactured by Promega) having the promoter of SV40 virus, and a plasmid pCAT-Basic (manufactured by Promega) containing no promoters, gene introduction was performed on the lung cancer cells QG-56, the neuroblastoma NGP, and the normal human fibroblasts MRC-5 that were being cultured in a DMEM (Sigma) supplemented with 10% bovine fetal serum.

Article Title: Use of the human MDR1 promoter for heat-inducible expression of therapeutic genes.
Article Snippet: The promoter of the human multidrug resistance gene (mdr1) harbors stress-responsive elements, which can be induced e.g., by heat or cytostatic drugs.. In previous studies the drug-responsiveness of the mdr1 promoter was successfully used for the drug-inducible expression of the human TNFgene in vitro and in vivo.. Beside the drug-responsive elements of the mdr1 promoter, heat-shock responsive elements have also been identified, which could be exploited for construction of heat-inducible expression vectors.

Article Title: Effect of Adenovirus E1A on ICAM-1 Promoter Activity in Human Alveolar and Bronchial Epithelial Cells
Article Snippet: The plasmid pCAT-Control (Promega, Madison, WI), which contains the SV40 promoter and enhancer sequences placed 5’ of the CAT gene, was used as a positive control for CAT expression and as a means of monitoring differences in transfection efficiency between cell lines.

Activity Assay:

Article Title: Understanding oligonucleotide-mediated inhibition of gene expression in Xenopus laevis oocytes
Article Snippet: Preparation of plasmid pCAT-control was purchased from Promega Biotech (Madison, WI). pCAT-control and all other plasmids used were amplified and purified after transformation into Escherichia coli strain DH5-α, using a Qiagen (Chatsworth, CA) midi preparation kit.

Article Title: Suppression of interleukin-1beta-induced nitric-oxide synthase promoter/enhancer activity by transforming growth factor-beta1 in vascular smooth muscle cells. Evidence for mechanisms other than NF-kappaB.
Article Snippet: Plasmid pCAT-Control contained the prokaryotic chloramphenicol acetyltransferase (CAT) gene (Promega) driven by the SV40 promoter and enhancer.

Article Title: Tumor specific promoters of the midkine gene that allow for selective expression in P53-inactivated cells
Article Snippet: Examination on Transcription Activity by Tumor-specific Promoters Derived From the Midkine Gene Using the above plasmid, or a plasmid pCAT-Control (manufactured by Promega) having the promoter of SV40 virus, and a plasmid pCAT-Basic (manufactured by Promega) containing no promoters, gene introduction was performed on the lung cancer cells QG-56, the neuroblastoma NGP, and the normal human fibroblasts MRC-5 that were being cultured in a DMEM (Sigma) supplemented with 10% bovine fetal serum.

Article Title: Use of the human MDR1 promoter for heat-inducible expression of therapeutic genes.
Article Snippet: The promoter of the human multidrug resistance gene (mdr1) harbors stress-responsive elements, which can be induced e.g., by heat or cytostatic drugs.. In previous studies the drug-responsiveness of the mdr1 promoter was successfully used for the drug-inducible expression of the human TNFgene in vitro and in vivo.. Beside the drug-responsive elements of the mdr1 promoter, heat-shock responsive elements have also been identified, which could be exploited for construction of heat-inducible expression vectors.

Article Title: Effect of Adenovirus E1A on ICAM-1 Promoter Activity in Human Alveolar and Bronchial Epithelial Cells
Article Snippet: The plasmid pCAT-Control (Promega, Madison, WI), which contains the SV40 promoter and enhancer sequences placed 5’ of the CAT gene, was used as a positive control for CAT expression and as a means of monitoring differences in transfection efficiency between cell lines.

Derivative Assay:

Article Title: Understanding oligonucleotide-mediated inhibition of gene expression in Xenopus laevis oocytes
Article Snippet: Preparation of plasmid pCAT-control was purchased from Promega Biotech (Madison, WI). pCAT-control and all other plasmids used were amplified and purified after transformation into Escherichia coli strain DH5-α, using a Qiagen (Chatsworth, CA) midi preparation kit.

Article Title: Suppression of interleukin-1beta-induced nitric-oxide synthase promoter/enhancer activity by transforming growth factor-beta1 in vascular smooth muscle cells. Evidence for mechanisms other than NF-kappaB.
Article Snippet: Plasmid pCAT-Control contained the prokaryotic chloramphenicol acetyltransferase (CAT) gene (Promega) driven by the SV40 promoter and enhancer.

Article Title: Tumor specific promoters of the midkine gene that allow for selective expression in P53-inactivated cells
Article Snippet: Examination on Transcription Activity by Tumor-specific Promoters Derived From the Midkine Gene Using the above plasmid, or a plasmid pCAT-Control (manufactured by Promega) having the promoter of SV40 virus, and a plasmid pCAT-Basic (manufactured by Promega) containing no promoters, gene introduction was performed on the lung cancer cells QG-56, the neuroblastoma NGP, and the normal human fibroblasts MRC-5 that were being cultured in a DMEM (Sigma) supplemented with 10% bovine fetal serum.

Article Title: Use of the human MDR1 promoter for heat-inducible expression of therapeutic genes.
Article Snippet: The promoter of the human multidrug resistance gene (mdr1) harbors stress-responsive elements, which can be induced e.g., by heat or cytostatic drugs.. In previous studies the drug-responsiveness of the mdr1 promoter was successfully used for the drug-inducible expression of the human TNFgene in vitro and in vivo.. Beside the drug-responsive elements of the mdr1 promoter, heat-shock responsive elements have also been identified, which could be exploited for construction of heat-inducible expression vectors.

Article Title: Effect of Adenovirus E1A on ICAM-1 Promoter Activity in Human Alveolar and Bronchial Epithelial Cells
Article Snippet: The plasmid pCAT-Control (Promega, Madison, WI), which contains the SV40 promoter and enhancer sequences placed 5’ of the CAT gene, was used as a positive control for CAT expression and as a means of monitoring differences in transfection efficiency between cell lines.

Virus:

Article Title: Understanding oligonucleotide-mediated inhibition of gene expression in Xenopus laevis oocytes
Article Snippet: Preparation of plasmid pCAT-control was purchased from Promega Biotech (Madison, WI). pCAT-control and all other plasmids used were amplified and purified after transformation into Escherichia coli strain DH5-α, using a Qiagen (Chatsworth, CA) midi preparation kit.

Article Title: Suppression of interleukin-1beta-induced nitric-oxide synthase promoter/enhancer activity by transforming growth factor-beta1 in vascular smooth muscle cells. Evidence for mechanisms other than NF-kappaB.
Article Snippet: Plasmid pCAT-Control contained the prokaryotic chloramphenicol acetyltransferase (CAT) gene (Promega) driven by the SV40 promoter and enhancer.

Article Title: Tumor specific promoters of the midkine gene that allow for selective expression in P53-inactivated cells
Article Snippet: Examination on Transcription Activity by Tumor-specific Promoters Derived From the Midkine Gene Using the above plasmid, or a plasmid pCAT-Control (manufactured by Promega) having the promoter of SV40 virus, and a plasmid pCAT-Basic (manufactured by Promega) containing no promoters, gene introduction was performed on the lung cancer cells QG-56, the neuroblastoma NGP, and the normal human fibroblasts MRC-5 that were being cultured in a DMEM (Sigma) supplemented with 10% bovine fetal serum.

Article Title: Use of the human MDR1 promoter for heat-inducible expression of therapeutic genes.
Article Snippet: The promoter of the human multidrug resistance gene (mdr1) harbors stress-responsive elements, which can be induced e.g., by heat or cytostatic drugs.. In previous studies the drug-responsiveness of the mdr1 promoter was successfully used for the drug-inducible expression of the human TNFgene in vitro and in vivo.. Beside the drug-responsive elements of the mdr1 promoter, heat-shock responsive elements have also been identified, which could be exploited for construction of heat-inducible expression vectors.

Article Title: Effect of Adenovirus E1A on ICAM-1 Promoter Activity in Human Alveolar and Bronchial Epithelial Cells
Article Snippet: The plasmid pCAT-Control (Promega, Madison, WI), which contains the SV40 promoter and enhancer sequences placed 5’ of the CAT gene, was used as a positive control for CAT expression and as a means of monitoring differences in transfection efficiency between cell lines.

Cell Culture:

Article Title: Understanding oligonucleotide-mediated inhibition of gene expression in Xenopus laevis oocytes
Article Snippet: Preparation of plasmid pCAT-control was purchased from Promega Biotech (Madison, WI). pCAT-control and all other plasmids used were amplified and purified after transformation into Escherichia coli strain DH5-α, using a Qiagen (Chatsworth, CA) midi preparation kit.

Article Title: Suppression of interleukin-1beta-induced nitric-oxide synthase promoter/enhancer activity by transforming growth factor-beta1 in vascular smooth muscle cells. Evidence for mechanisms other than NF-kappaB.
Article Snippet: Plasmid pCAT-Control contained the prokaryotic chloramphenicol acetyltransferase (CAT) gene (Promega) driven by the SV40 promoter and enhancer.

Article Title: Tumor specific promoters of the midkine gene that allow for selective expression in P53-inactivated cells
Article Snippet: Examination on Transcription Activity by Tumor-specific Promoters Derived From the Midkine Gene Using the above plasmid, or a plasmid pCAT-Control (manufactured by Promega) having the promoter of SV40 virus, and a plasmid pCAT-Basic (manufactured by Promega) containing no promoters, gene introduction was performed on the lung cancer cells QG-56, the neuroblastoma NGP, and the normal human fibroblasts MRC-5 that were being cultured in a DMEM (Sigma) supplemented with 10% bovine fetal serum.

Article Title: Use of the human MDR1 promoter for heat-inducible expression of therapeutic genes.
Article Snippet: The promoter of the human multidrug resistance gene (mdr1) harbors stress-responsive elements, which can be induced e.g., by heat or cytostatic drugs.. In previous studies the drug-responsiveness of the mdr1 promoter was successfully used for the drug-inducible expression of the human TNFgene in vitro and in vivo.. Beside the drug-responsive elements of the mdr1 promoter, heat-shock responsive elements have also been identified, which could be exploited for construction of heat-inducible expression vectors.

Article Title: Effect of Adenovirus E1A on ICAM-1 Promoter Activity in Human Alveolar and Bronchial Epithelial Cells
Article Snippet: The plasmid pCAT-Control (Promega, Madison, WI), which contains the SV40 promoter and enhancer sequences placed 5’ of the CAT gene, was used as a positive control for CAT expression and as a means of monitoring differences in transfection efficiency between cell lines.

Sequencing:

Article Title: Understanding oligonucleotide-mediated inhibition of gene expression in Xenopus laevis oocytes
Article Snippet: Preparation of plasmid pCAT-control was purchased from Promega Biotech (Madison, WI). pCAT-control and all other plasmids used were amplified and purified after transformation into Escherichia coli strain DH5-α, using a Qiagen (Chatsworth, CA) midi preparation kit.

Article Title: Suppression of interleukin-1beta-induced nitric-oxide synthase promoter/enhancer activity by transforming growth factor-beta1 in vascular smooth muscle cells. Evidence for mechanisms other than NF-kappaB.
Article Snippet: Plasmid pCAT-Control contained the prokaryotic chloramphenicol acetyltransferase (CAT) gene (Promega) driven by the SV40 promoter and enhancer.

Article Title: Tumor specific promoters of the midkine gene that allow for selective expression in P53-inactivated cells
Article Snippet: Examination on Transcription Activity by Tumor-specific Promoters Derived From the Midkine Gene Using the above plasmid, or a plasmid pCAT-Control (manufactured by Promega) having the promoter of SV40 virus, and a plasmid pCAT-Basic (manufactured by Promega) containing no promoters, gene introduction was performed on the lung cancer cells QG-56, the neuroblastoma NGP, and the normal human fibroblasts MRC-5 that were being cultured in a DMEM (Sigma) supplemented with 10% bovine fetal serum.

Article Title: Use of the human MDR1 promoter for heat-inducible expression of therapeutic genes.
Article Snippet: The promoter of the human multidrug resistance gene (mdr1) harbors stress-responsive elements, which can be induced e.g., by heat or cytostatic drugs.. In previous studies the drug-responsiveness of the mdr1 promoter was successfully used for the drug-inducible expression of the human TNFgene in vitro and in vivo.. Beside the drug-responsive elements of the mdr1 promoter, heat-shock responsive elements have also been identified, which could be exploited for construction of heat-inducible expression vectors.

Article Title: Effect of Adenovirus E1A on ICAM-1 Promoter Activity in Human Alveolar and Bronchial Epithelial Cells
Article Snippet: The plasmid pCAT-Control (Promega, Madison, WI), which contains the SV40 promoter and enhancer sequences placed 5’ of the CAT gene, was used as a positive control for CAT expression and as a means of monitoring differences in transfection efficiency between cell lines.

Amplification:

Article Title: Understanding oligonucleotide-mediated inhibition of gene expression in Xenopus laevis oocytes
Article Snippet: Preparation of plasmid pCAT-control was purchased from Promega Biotech (Madison, WI). pCAT-control and all other plasmids used were amplified and purified after transformation into Escherichia coli strain DH5-α, using a Qiagen (Chatsworth, CA) midi preparation kit.

Article Title: Suppression of interleukin-1beta-induced nitric-oxide synthase promoter/enhancer activity by transforming growth factor-beta1 in vascular smooth muscle cells. Evidence for mechanisms other than NF-kappaB.
Article Snippet: Plasmid pCAT-Control contained the prokaryotic chloramphenicol acetyltransferase (CAT) gene (Promega) driven by the SV40 promoter and enhancer.

Article Title: Tumor specific promoters of the midkine gene that allow for selective expression in P53-inactivated cells
Article Snippet: Examination on Transcription Activity by Tumor-specific Promoters Derived From the Midkine Gene Using the above plasmid, or a plasmid pCAT-Control (manufactured by Promega) having the promoter of SV40 virus, and a plasmid pCAT-Basic (manufactured by Promega) containing no promoters, gene introduction was performed on the lung cancer cells QG-56, the neuroblastoma NGP, and the normal human fibroblasts MRC-5 that were being cultured in a DMEM (Sigma) supplemented with 10% bovine fetal serum.

Article Title: Use of the human MDR1 promoter for heat-inducible expression of therapeutic genes.
Article Snippet: The promoter of the human multidrug resistance gene (mdr1) harbors stress-responsive elements, which can be induced e.g., by heat or cytostatic drugs.. In previous studies the drug-responsiveness of the mdr1 promoter was successfully used for the drug-inducible expression of the human TNFgene in vitro and in vivo.. Beside the drug-responsive elements of the mdr1 promoter, heat-shock responsive elements have also been identified, which could be exploited for construction of heat-inducible expression vectors.

Article Title: Effect of Adenovirus E1A on ICAM-1 Promoter Activity in Human Alveolar and Bronchial Epithelial Cells
Article Snippet: The plasmid pCAT-Control (Promega, Madison, WI), which contains the SV40 promoter and enhancer sequences placed 5’ of the CAT gene, was used as a positive control for CAT expression and as a means of monitoring differences in transfection efficiency between cell lines.

Purification:

Article Title: Understanding oligonucleotide-mediated inhibition of gene expression in Xenopus laevis oocytes
Article Snippet: Preparation of plasmid pCAT-control was purchased from Promega Biotech (Madison, WI). pCAT-control and all other plasmids used were amplified and purified after transformation into Escherichia coli strain DH5-α, using a Qiagen (Chatsworth, CA) midi preparation kit.

Article Title: Suppression of interleukin-1beta-induced nitric-oxide synthase promoter/enhancer activity by transforming growth factor-beta1 in vascular smooth muscle cells. Evidence for mechanisms other than NF-kappaB.
Article Snippet: Plasmid pCAT-Control contained the prokaryotic chloramphenicol acetyltransferase (CAT) gene (Promega) driven by the SV40 promoter and enhancer.

Article Title: Tumor specific promoters of the midkine gene that allow for selective expression in P53-inactivated cells
Article Snippet: Examination on Transcription Activity by Tumor-specific Promoters Derived From the Midkine Gene Using the above plasmid, or a plasmid pCAT-Control (manufactured by Promega) having the promoter of SV40 virus, and a plasmid pCAT-Basic (manufactured by Promega) containing no promoters, gene introduction was performed on the lung cancer cells QG-56, the neuroblastoma NGP, and the normal human fibroblasts MRC-5 that were being cultured in a DMEM (Sigma) supplemented with 10% bovine fetal serum.

Article Title: Use of the human MDR1 promoter for heat-inducible expression of therapeutic genes.
Article Snippet: The promoter of the human multidrug resistance gene (mdr1) harbors stress-responsive elements, which can be induced e.g., by heat or cytostatic drugs.. In previous studies the drug-responsiveness of the mdr1 promoter was successfully used for the drug-inducible expression of the human TNFgene in vitro and in vivo.. Beside the drug-responsive elements of the mdr1 promoter, heat-shock responsive elements have also been identified, which could be exploited for construction of heat-inducible expression vectors.

Article Title: Effect of Adenovirus E1A on ICAM-1 Promoter Activity in Human Alveolar and Bronchial Epithelial Cells
Article Snippet: The plasmid pCAT-Control (Promega, Madison, WI), which contains the SV40 promoter and enhancer sequences placed 5’ of the CAT gene, was used as a positive control for CAT expression and as a means of monitoring differences in transfection efficiency between cell lines.

Transformation Assay:

Article Title: Understanding oligonucleotide-mediated inhibition of gene expression in Xenopus laevis oocytes
Article Snippet: Preparation of plasmid pCAT-control was purchased from Promega Biotech (Madison, WI). pCAT-control and all other plasmids used were amplified and purified after transformation into Escherichia coli strain DH5-α, using a Qiagen (Chatsworth, CA) midi preparation kit.

Article Title: Suppression of interleukin-1beta-induced nitric-oxide synthase promoter/enhancer activity by transforming growth factor-beta1 in vascular smooth muscle cells. Evidence for mechanisms other than NF-kappaB.
Article Snippet: Plasmid pCAT-Control contained the prokaryotic chloramphenicol acetyltransferase (CAT) gene (Promega) driven by the SV40 promoter and enhancer.

Article Title: Tumor specific promoters of the midkine gene that allow for selective expression in P53-inactivated cells
Article Snippet: Examination on Transcription Activity by Tumor-specific Promoters Derived From the Midkine Gene Using the above plasmid, or a plasmid pCAT-Control (manufactured by Promega) having the promoter of SV40 virus, and a plasmid pCAT-Basic (manufactured by Promega) containing no promoters, gene introduction was performed on the lung cancer cells QG-56, the neuroblastoma NGP, and the normal human fibroblasts MRC-5 that were being cultured in a DMEM (Sigma) supplemented with 10% bovine fetal serum.

Article Title: Use of the human MDR1 promoter for heat-inducible expression of therapeutic genes.
Article Snippet: The promoter of the human multidrug resistance gene (mdr1) harbors stress-responsive elements, which can be induced e.g., by heat or cytostatic drugs.. In previous studies the drug-responsiveness of the mdr1 promoter was successfully used for the drug-inducible expression of the human TNFgene in vitro and in vivo.. Beside the drug-responsive elements of the mdr1 promoter, heat-shock responsive elements have also been identified, which could be exploited for construction of heat-inducible expression vectors.

Article Title: Effect of Adenovirus E1A on ICAM-1 Promoter Activity in Human Alveolar and Bronchial Epithelial Cells
Article Snippet: The plasmid pCAT-Control (Promega, Madison, WI), which contains the SV40 promoter and enhancer sequences placed 5’ of the CAT gene, was used as a positive control for CAT expression and as a means of monitoring differences in transfection efficiency between cell lines.



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